If you're using a Canon digital camera, you can only read the ISO in it's ZoomBrowser software.
Hmmmmn, obviously not all Canon cameras fall into that statement Gail. As shown above the Canon 20D info can be read in IExif.

Note: A quick check in Picasa also shows the some EXIF data (including ISO) in .jpg files converted from RAW (though no EXIF shows for the RAW file.